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aav1 hsyn cre wpre hgh  (Addgene inc)


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    Structured Review

    Addgene inc aav1 hsyn cre wpre hgh
    Aav1 Hsyn Cre Wpre Hgh, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav1+hsyn+cre+wpre+hgh/pENN%2EAAV%2EhSyn%2ECre%2EWPRE%2EhGH+(Plasmid+%23105553)/bio_rxiv__64898__2026__03__27__714842-215-5-6
    Average 96 stars, based on 308 article reviews
    aav1 hsyn cre wpre hgh - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Microinjection:

    Article Title: Hypothalamic Orexinergic Neurons Projecting to the Mesencephalic Locomotor Region Are Activated by Voluntary Wheel Running Exercise in Rats
    Article Snippet: Anterograde transsynaptic tagging to label MLR neurons postsynaptic to hypothalamic neurons Male SD rats ( n = 4, 9–12 weeks, 347–486g) were anesthetized via inhalation of 5% isoflurane in oxygen followed by subcutaneous administration of a combined anesthetic (0.15 mg/kg of medetomidine hydrochloride, 2.0 mg/kg of midazolam, 2.5 mg/kg of butorphanol) and were then placed in a stereotaxic frame (SR‐6R, Narishige, Tokyo, Japan). .. With a calibrated pressure microinjection system (Nanoject II, Drummond Scientific Co., Broomall, PA), AAV1-hsyn-Cre-WPRE-hGH (184 nL) (donated by Wilson JM: Addgene Cat # 105553-AAV1) was injected unilaterally into the PeFA (3.0–3.1 mm caudal, 1.6 mm lateral, and 8.8–9.1 mm ventral to the bregma). ..

    Injection:

    Article Title: Hypothalamic Orexinergic Neurons Projecting to the Mesencephalic Locomotor Region Are Activated by Voluntary Wheel Running Exercise in Rats
    Article Snippet: Anterograde transsynaptic tagging to label MLR neurons postsynaptic to hypothalamic neurons Male SD rats ( n = 4, 9–12 weeks, 347–486g) were anesthetized via inhalation of 5% isoflurane in oxygen followed by subcutaneous administration of a combined anesthetic (0.15 mg/kg of medetomidine hydrochloride, 2.0 mg/kg of midazolam, 2.5 mg/kg of butorphanol) and were then placed in a stereotaxic frame (SR‐6R, Narishige, Tokyo, Japan). .. With a calibrated pressure microinjection system (Nanoject II, Drummond Scientific Co., Broomall, PA), AAV1-hsyn-Cre-WPRE-hGH (184 nL) (donated by Wilson JM: Addgene Cat # 105553-AAV1) was injected unilaterally into the PeFA (3.0–3.1 mm caudal, 1.6 mm lateral, and 8.8–9.1 mm ventral to the bregma). ..

    Article Title: Direct piriform-to-auditory cortical projections shape auditory-olfactory integration
    Article Snippet: To label ACx-projecting neurons in Pir in Ai14 mice, we injected AAVrg-hSyn-Cre-WPRE-hGH (Addgene #105553-AAVrg; RRID:Addgene_105553; 150-250 nL) into left ACx. .. To label Pir-recipient neurons in ACx in Ai14 mice, we injected AAV1-hSyn-Cre-WPRE-hGH (Addgene #105553-AAV1; RRID:Addgene_105553; 150-180 nL) into left Pir (3.9 mm ML, -1.5 mm AP, -4.00 DV). .. Viruses were delivered through 30-40 μm diameter glass micropipettes connected to a syringe pump (Harvard Apparatus Pump 11 Elite; 60 nL/min infusion rate).

    Article Title: Paratenial thalamus engages in reciprocal and broadcast circuits with the prefrontal cortex
    Article Snippet: Anterograde Cre-dependent anterograde labelling was achieved using AAV1-EF1a-DIO-eYFP-WPRE.hGH (Addgene #27056-AAV1, titer = 2.5E+13 GC/ml) for anatomical tracing, and AAV1.EF1a.DIO.hChR2(H134R)-eYFP.WPRE.hGH (Addgene #20298-AAV1, titer = 2.3E+13 GC/ml) for optogenetic stimulation. .. For transsynaptic labeling of PT, AAV1-hSyn-Cre-WPRE.hGH (Addgene #105553-AAV1, titer = 1.9E+13 GC/ml) was injected into mPFC, and AAV1.EF1a.DIO.hChR2(H134R)-eYFP.WPRE.hGH was injected into PT. ..

    Recombinant:

    Article Title: A parabrachial hub for need-state control of enduring pain
    Article Snippet: .. The following recombinant adeno-associated virus (rAAV) vectors were used: AAV5.hSyn.DIO.hM3D(Gq).mCherry (Addgene 44361 from B. Roth , titre: 2.1 × 10 13 genome copies (GC) per ml), AAV8.hSyn.DIO.hM4D(Gi) (Addgene 44362 from B. Roth , titre: 2.2 × 10 13 GC ml −1 ), AAV2.FLEX.DTR.GFP (Addgene 124364 from E. Azim and T. Jessell , titre: 2.0 × 10 13 GC ml −1 ), AAV8.hSyn.fDIO.hm3D(Gq).mCherry.WPREpA (Addgene 154868 from U. Gether), AAV8.hSyn.DIO.mCherry (Addgene 50459 from B. Roth, titre: 3.6 × 10 13 GC ml −1 ), AAV1.Syn.Flex.GCaMP6s.WPRE.SV40 (Addgene 100845 from D. Kim and the GENIE project , titre: 4.2 × 10 13 GC ml −1 ), AAV1.hSynapsin1.axon.GCaMP6s (Addgene 111262 from L. Tian , titre: 4.1 × 10 13 GC ml −1 ), AAV1.syn.FLEX.splitTVA.EGFP.tTA (Addgene 100798 from I. Wickersham , titre: 1.2 × 10 12 GC ml −1 ), AAV1.TREtight.mTagBFP2.B19G (Addgene 100799 from I. Wickersham , titre: 1 × 10 13 GC ml −1 ), pSADdeltaG.mCherry (Addgene 32636 from E. Callaway , titre: 3.8 × 10 12 GC ml −1 ), AAV1.CBA.DO(FAS).GCaMP6s (Addgene plasmid 110135 from B. Sabatini , packaged by Vigene Biosciences, titre: 4.1 × 10 13 GC ml −1 ), AAV1.hSyn.Cre.WPRE.hGH (Addgene 105553 from J. M. Wilson, 2.5 × 10 13 GC ml −1 ), AAV1.CAG.FLEX.EGFP (Addgene 59331 from I. Wickersham), AAV1.hSyn.GRAB_NPY1.0 (Addgene 208676 from Y. Li packaged by Neurotools), AAV1.CAG.GFP.U6.m.Npy1r.shRNA (Vector Biolabs), AAV1-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (Addgene 20298 from K. Deisseroth, titre: 2.1 ×10 13 GC ml –1 ) and AAV1.FLEX.tdTomato (Addgene 28306 from E. Boyden, titre: 3.1 × 10 13 GC ml −1 ). ..

    Virus:

    Article Title: A parabrachial hub for need-state control of enduring pain
    Article Snippet: .. The following recombinant adeno-associated virus (rAAV) vectors were used: AAV5.hSyn.DIO.hM3D(Gq).mCherry (Addgene 44361 from B. Roth , titre: 2.1 × 10 13 genome copies (GC) per ml), AAV8.hSyn.DIO.hM4D(Gi) (Addgene 44362 from B. Roth , titre: 2.2 × 10 13 GC ml −1 ), AAV2.FLEX.DTR.GFP (Addgene 124364 from E. Azim and T. Jessell , titre: 2.0 × 10 13 GC ml −1 ), AAV8.hSyn.fDIO.hm3D(Gq).mCherry.WPREpA (Addgene 154868 from U. Gether), AAV8.hSyn.DIO.mCherry (Addgene 50459 from B. Roth, titre: 3.6 × 10 13 GC ml −1 ), AAV1.Syn.Flex.GCaMP6s.WPRE.SV40 (Addgene 100845 from D. Kim and the GENIE project , titre: 4.2 × 10 13 GC ml −1 ), AAV1.hSynapsin1.axon.GCaMP6s (Addgene 111262 from L. Tian , titre: 4.1 × 10 13 GC ml −1 ), AAV1.syn.FLEX.splitTVA.EGFP.tTA (Addgene 100798 from I. Wickersham , titre: 1.2 × 10 12 GC ml −1 ), AAV1.TREtight.mTagBFP2.B19G (Addgene 100799 from I. Wickersham , titre: 1 × 10 13 GC ml −1 ), pSADdeltaG.mCherry (Addgene 32636 from E. Callaway , titre: 3.8 × 10 12 GC ml −1 ), AAV1.CBA.DO(FAS).GCaMP6s (Addgene plasmid 110135 from B. Sabatini , packaged by Vigene Biosciences, titre: 4.1 × 10 13 GC ml −1 ), AAV1.hSyn.Cre.WPRE.hGH (Addgene 105553 from J. M. Wilson, 2.5 × 10 13 GC ml −1 ), AAV1.CAG.FLEX.EGFP (Addgene 59331 from I. Wickersham), AAV1.hSyn.GRAB_NPY1.0 (Addgene 208676 from Y. Li packaged by Neurotools), AAV1.CAG.GFP.U6.m.Npy1r.shRNA (Vector Biolabs), AAV1-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (Addgene 20298 from K. Deisseroth, titre: 2.1 ×10 13 GC ml –1 ) and AAV1.FLEX.tdTomato (Addgene 28306 from E. Boyden, titre: 3.1 × 10 13 GC ml −1 ). ..

    Plasmid Preparation:

    Article Title: A parabrachial hub for need-state control of enduring pain
    Article Snippet: .. The following recombinant adeno-associated virus (rAAV) vectors were used: AAV5.hSyn.DIO.hM3D(Gq).mCherry (Addgene 44361 from B. Roth , titre: 2.1 × 10 13 genome copies (GC) per ml), AAV8.hSyn.DIO.hM4D(Gi) (Addgene 44362 from B. Roth , titre: 2.2 × 10 13 GC ml −1 ), AAV2.FLEX.DTR.GFP (Addgene 124364 from E. Azim and T. Jessell , titre: 2.0 × 10 13 GC ml −1 ), AAV8.hSyn.fDIO.hm3D(Gq).mCherry.WPREpA (Addgene 154868 from U. Gether), AAV8.hSyn.DIO.mCherry (Addgene 50459 from B. Roth, titre: 3.6 × 10 13 GC ml −1 ), AAV1.Syn.Flex.GCaMP6s.WPRE.SV40 (Addgene 100845 from D. Kim and the GENIE project , titre: 4.2 × 10 13 GC ml −1 ), AAV1.hSynapsin1.axon.GCaMP6s (Addgene 111262 from L. Tian , titre: 4.1 × 10 13 GC ml −1 ), AAV1.syn.FLEX.splitTVA.EGFP.tTA (Addgene 100798 from I. Wickersham , titre: 1.2 × 10 12 GC ml −1 ), AAV1.TREtight.mTagBFP2.B19G (Addgene 100799 from I. Wickersham , titre: 1 × 10 13 GC ml −1 ), pSADdeltaG.mCherry (Addgene 32636 from E. Callaway , titre: 3.8 × 10 12 GC ml −1 ), AAV1.CBA.DO(FAS).GCaMP6s (Addgene plasmid 110135 from B. Sabatini , packaged by Vigene Biosciences, titre: 4.1 × 10 13 GC ml −1 ), AAV1.hSyn.Cre.WPRE.hGH (Addgene 105553 from J. M. Wilson, 2.5 × 10 13 GC ml −1 ), AAV1.CAG.FLEX.EGFP (Addgene 59331 from I. Wickersham), AAV1.hSyn.GRAB_NPY1.0 (Addgene 208676 from Y. Li packaged by Neurotools), AAV1.CAG.GFP.U6.m.Npy1r.shRNA (Vector Biolabs), AAV1-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (Addgene 20298 from K. Deisseroth, titre: 2.1 ×10 13 GC ml –1 ) and AAV1.FLEX.tdTomato (Addgene 28306 from E. Boyden, titre: 3.1 × 10 13 GC ml −1 ). ..

    Article Title: A parabrachial hub for the prioritization of survival behavior
    Article Snippet: .. The following rAAV vectors were used: AAV5.hSyn.DIO.hM3D(Gq).mCherry (Addgene 44361 from Bryan Roth , titer: 2.1e13 GC/mL), AAV8.hSyn.DIO.hM4D(Gi) (Addgene 44362 from Bryan Roth , titer: 2.2e13 GC/mL), AAV2.FLEX.DTR.GFP (Addgene 124364 from Eiman Azim and Thomas Jessel , titer: 2.0e13 GC/mL), AAV8.hSyn.DIO.mCherry (Addgene 50459 from Bryan Roth, titer: 3.6e13 GC/mL), AAV1.Syn.Flex.GCaMP6s.WPRE.SV40 (Addgene 100845 from Douglas Kim and the GENIE project , titer: 4.2e13 GC/mL), AAV1.hSynapsin1.axon.GCaMP6s (Addgene 111262 from Lin Tian , titer: 4.1e13 GC/mL), AAV1.syn.FLEX.splitTVA.EGFP.tTA (Addgene 100798 from Ian Wickersham , titer: 1.2e12 GC/mL), AAV1.TREtight.mTagBFP2.B19G (Addgene 100799 from Ian Wickersham , titer: 1e13 GC/mL), pSADdeltaG.mCherry (Addgene 32636 from Edward Callaway , titer: 3.8e12 GC/mL), AAV1.CBA.DO(FAS).GCaMP6s (Addgene plasmid 110135 from Bernardo Sabatini , packaged by Vigene Biosciences, titer: 4.1e13 GC/mL), AAV1.hSyn.Cre.WPRE.hGH (Addgene 105553 from James M. Wilson, 2.5e13 GC.mL) and AAV1.FLEX.tdTomato (Addgene 28306 from Edward Boyden, titer: 3.1e13 GC/mL). ..

    Labeling:

    Article Title: Paratenial thalamus engages in reciprocal and broadcast circuits with the prefrontal cortex
    Article Snippet: Anterograde Cre-dependent anterograde labelling was achieved using AAV1-EF1a-DIO-eYFP-WPRE.hGH (Addgene #27056-AAV1, titer = 2.5E+13 GC/ml) for anatomical tracing, and AAV1.EF1a.DIO.hChR2(H134R)-eYFP.WPRE.hGH (Addgene #20298-AAV1, titer = 2.3E+13 GC/ml) for optogenetic stimulation. .. For transsynaptic labeling of PT, AAV1-hSyn-Cre-WPRE.hGH (Addgene #105553-AAV1, titer = 1.9E+13 GC/ml) was injected into mPFC, and AAV1.EF1a.DIO.hChR2(H134R)-eYFP.WPRE.hGH was injected into PT. ..



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